Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean. Description and images of Krameria lappacea (), a native Chilean plant, provided by the supplier of native exotic Chilean seeds and organizer of plant watching. Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean South America, and.
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The developed HPLC method enables the quantification of twelve active lignan derivatives in the roots, and is also suitable for the determination of lpapacea constituents in Tinctura Ratanhiae. Root preparations of Krameria lappacea Dombey Burdet et Simpson are traditionally used against oropharyngeal inflammation. Supplementary data Click here to view. The root bark contains an almost insoluble free red substance called ratanhia red.
The detector signal was linear in the tested range with a correlation coefficient higher than 0. Wikipedia articles incorporating a citation from the Encyclopaedia Britannica with Wikisource reference Articles with ‘species’ microformats Wikipedia articles incorporating a citation from the Encyclopedia Americana with a Wikisource reference Commons category link from Wikidata.
Three root samples RR-1 to RR-3 and four ethanolic tinctures T-1 to T-43 of them were purchased in pharmacies around Austria, were analyzed during this study, the obtained results are illustrated in Table 3.
The major problem in the development of a HPLC method for the simultaneous detection of all active lignan derivatives was the structural similarity of the lapacea. The amount found in the samples was calculated as percent of the initial weight of the dried roots. These pharmacological findings make a quality assessment of Ratanhiae radix and its preparations with an adequate analytical method with focus on the class of lignan derivatives, necessary.
Compound 7 was isolated as following: They are perennial shrubs which act as root parasites on other plants. Author information Article notes Copyright and License information Disclaimer. Abstract Root preparations of Krameria lappacea Dombey Burdet et Simpson are traditionally used against oropharyngeal inflammation. The topical anti-inflammatory properties of the secondary metabolites were comparable to the drug indomethacin ID 50 value 0.
Only for compound 4 LOD Germplasm Resources Information Network. One problem emerged at the beginning of sample analysis when evaluating purity of all analytes by the use of the UV purity function of the used HPLC software: Krameria is the only genus in the Krameriaceae family, of which any of the approximately 18 species  are commonly known as rhatanyratany or rattany.
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In order to check if this extraction protocol is exhaustive, another 5 cycles after the normal extraction protocol were krsmeria and the obtained extract analyzed by HPLC.
Krameria lappacea – Useful Tropical Plants
None of the compounds 1 — 12 could rkameria quantified since their content was below the LOQ and even below the LOD, indicating an exhaustive extraction. Method validation Suitability of the developed method for the quantification of the major lignan derivatives in Ratanhiae radix and Tinctura Ratanhiae can be deduced from several analytical parameters.
Optimal results were obtained by applying the tincture samples directly on top of a polyamide column followed by elution with methanol. This issue could be explained by the shorter maceration time 3 dayssince regular Tinctura Ratanhiae is prepared by a double maceration over a period of two weeks. National Center for Biotechnology InformationU. HPLC—MS experiments in positive and negative electrospray ionization mode confirmed identity and peak purity of analytes.
Attempts to improve the resolution with the aid of additives, such as tetrahydrofuran, 1- and 2-propanol or tert-butylmethyl ether, or a variation of temperature showed no effect.
Solvent A was changed from 0. Therefore we developed an efficient and according to the ICH guidelines validated HPLC method for the quantification of the active constituents in the roots as well as in the ethanolic tincture. Isolation of ratanhine, a new dineolignan from the medicinal Ratanhiae Radix.
Chromatograms of the standard mix, a typical root and a tincture sample under optimized conditions are depicted in Fig. For quantification of compound 8 and 11 kfameria obtained solutions were further diluted with methanol 1: Following these conditions the lignan derivatives were exhaustively extracted but the yield of undesired tannins could be minimized.
Known amounts of the standard compounds were added either to the dry powdered root material, followed by extraction or were applied on the polyamide column together with the tincture. The quality of those complied with lalpacea Austrian Pharmacopoeia.
Antioxidant and photoprotective activity of a lipophilic extract containing neolignans from Krameria triandra roots. Following parameters were applied: Thus, the HPLC-method, developed in the present study, is a significant improvement and innovation, since it enables for the first time the quantitative analysis of lignan derivatives in Ratanhiae radix as well as in Tinctura Ratanhiae.
The drug was introduced into European medicine over years ago as a remedy against stomach aches, diarrhoea, menstrual problems, nose bleeds and oropharyngeal inflammation [2,3].
Krameria lappacea Images
The biological action of rhatany is caused by the astringent rhataniatannic acidwhich is similar to tannic acid. The flowers have glands called elaiophores which produce a lipid which is collected by bees of the la;pacea Centris as they pollinate the flowers. By comparing variations within the same days intra-day precisions were determined, by observing differences within the 3 days inter-day precision was deduced.
In order to accomplish a satisfactory separation, all parameters had to be carefully assessed. Open in lappacae separate window. Sample analysis Preliminary tests were performed in order to determine optimum sample preparation conditions for the root as well as the tincture samples.